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  • I need help with The DNA fragments separated on an agarose gel can be visualised after staining with:

The DNA fragments separated on an agarose gel can be visualised after staining with:

  • Option 1)

    Bromophenol blue

  • Option 2)

    Acetocarmine

  • Option 3)

    Aniline blue

  • Option 4)

    Ethidium Bromide

 

Answers (1)

best_answer

As discussed in

Ethidium Bromide -

The separated DNA fragments can be seen only after staining with compound known as Ethidium Bromide followed by exposure to U.V. radiation as bright orange coloured bands.

- wherein

Ehtidium bromide is a fluoroscent dye, which intercalate with  DNA molecules and flourish when exposed to UV light (302 nm).

 

 EtBr is used to stain DNA fragments


Option 1)

Bromophenol blue

Option is incorrect

Option 2)

Acetocarmine

Option is incorrect

Option 3)

Aniline blue

Option is incorrect

Option 4)

Ethidium Bromide

Option is correct

Posted by

prateek

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