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If you want to obtain precise information regarding the exact time and location of synthesis of new DNA, which of the following methods would be most effective for such a study?
Electron microscopy
Carbon dating
Isolating and extracting DNA after regular intervals and estimating DNA amount
Using radioactive precursors of nucleic acids.
Answers (1)
Using radioactive precursors of nucleic acids can indeed be an effective method for studying the synthesis of new DNA and obtaining precise information about the time and location of synthesis. Here's how it works:
Incorporation of Radioactive Precursors: Radioactive nucleotide precursors, such as radioactive isotopes of thymidine (e.g., tritiated thymidine or [3H]thymidine), can be added to the cell culture or organism under investigation. These radioactive precursors are incorporated into the newly synthesized DNA molecules during replication.
Detection of Radioactivity: Since the radioactive isotopes emit radiation, their presence in the newly synthesized DNA can be detected using various techniques. One commonly used method is autoradiography. The cells or tissues are exposed to a photographic film or an imaging plate, which records the emitted radiation as dark spots or lines on the film or plate. The intensity and location of the radioactive signals provide information about the rate and location of DNA synthesis.
Time Course Analysis: By exposing the cells or organisms to the radioactive precursors for different durations, it is possible to perform a time course analysis. This helps determine the exact time when DNA synthesis occurs. By analyzing samples taken at different time points, you can observe the progression of radioactivity incorporation and identify the stage of DNA synthesis.
Localization of DNA Synthesis: Depending on the specific research question, the study can be conducted at the cellular or tissue level. For cellular-level studies, cells can be labeled individually and examined under a microscope to identify which cells are actively synthesizing DNA. For tissue-level studies, sections of the tissue can be analyzed to determine the specific regions or cell types that are involved in DNA synthesis.
Hence, the correct answer is option 4.
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