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In PCR, assuming the ideal conditions, what is the potential level of amplification that can be achieved when performing 30 cycles of PCR to amplify a gene of interest?



Option: 1

0.1 million times

Option: 2

1.0 million times

Option: 3

1.0 billion times


Option: 4

1.0 trillion times

Answers (1)

  • PCR (Polymerase Chain Reaction) is a powerful technique used to amplify specific DNA sequences. During each cycle of PCR, the target DNA is replicated exponentially. Assuming ideal conditions and efficiency, the number of copies of the target DNA doubles with each cycle.
  • Since PCR amplifies exponentially, the total amplification after a given number of cycles can be calculated using the formula: \mathrm{Amplification = 2^{n}} (where n = number of cycles).
  • In this case, the question states that 30 cycles of PCR are performed. Therefore, the amplification can be calculated as follows:
  • Amplification = 230 = 1,073,741,824.
  • This means that after 30 cycles of PCR, the target DNA can potentially be amplified up to 1.0 billion times.
  • Hence, the correct answer is option (3).
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