Browse by Stream
QnA
Home
QnA
Go To Your Study Dashboard

Get Answers to all your Questions

header-bg qa
  • Home
  • Medical
  • In PCR, assuming the ideal conditions, what is the potential level of amplification that can be achieved when performing 30 cycles of PCR to amplify a gene of interest?

In PCR, assuming the ideal conditions, what is the potential level of amplification that can be achieved when performing 30 cycles of PCR to amplify a gene of interest?

 

 

Option: 1

0.1 million times


Option: 2

1.0 million times


Option: 3

1.0 billion times

 


Option: 4

1.0 trillion times


Answers (1)

best_answer
  • PCR (Polymerase Chain Reaction) is a powerful technique used to amplify specific DNA sequences. During each cycle of PCR, the target DNA is replicated exponentially. Assuming ideal conditions and efficiency, the number of copies of the target DNA doubles with each cycle.
  • Since PCR amplifies exponentially, the total amplification after a given number of cycles can be calculated using the formula: \mathrm{Amplification = 2^{n}} (where n = number of cycles).
  • In this case, the question states that 30 cycles of PCR are performed. Therefore, the amplification can be calculated as follows:
  • Amplification = 230 = 1,073,741,824.
  • This means that after 30 cycles of PCR, the target DNA can potentially be amplified up to 1.0 billion times.
  • Hence, the correct answer is option (3).
Posted by

Rishabh

View full answer

NEET 2024 Most scoring concepts

    Just Study 32% of the NEET syllabus and Score up to 100% marks

Similar Questions

Trending Articles/News

anam-khan

17 left in NEET toppers list 2024 as physics question strips 44 of top ranks
anam-khan

NEET Revised Result 2024: Joy and disappointment as all-India ranks change by thousands of places
anam-khan

NEET UG 2024 cut-offs drop for all categories; final scorecard out at neet.ntaonline.in

Latest Question