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In the process of DNA restriction, which specific target does each restriction enzyme inspect in a DNA sequence before cleaving it?


Option: 1

The minor groove of the DNA helix to produce sticky ends.

Option: 2

The anti-sense strand of DNA to produce sticky ends.

Option: 3

Specific points on both strands of the double helix in their sugar-phosphate backbones.

Option: 4

The mRNA molecule to remove exons.

Answers (1)


In the process of DNA restriction, each restriction enzyme inspects the DNA sequence and recognizes a specific target site or sequence. These enzymes typically recognize and bind to a specific sequence on the DNA, which is usually palindromic. Once the restriction enzyme identifies its target sequence, it cleaves the DNA at specific points within that sequence.

Option (2) is correct because restriction enzymes usually cleave the sense strand of the DNA, also known as the coding strand or non-template strand which produces the sticky ends. Sticky ends refer to the DNA fragments with single-stranded overhangs that can hybridize with complementary sequences, facilitating the insertion of foreign DNA fragments into the DNA molecule. Hence, the correct answer is option 2.


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