What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
The larger the fragment size, the farther it moves
The smaller the fragment size, the farther it moves
Positively charged fragments move to farther end
Negatively charged fragments do not move
As learnt in
Gel Electrophoresis -
This technique is used to separate DNA fragments on the basis of charge and size. Agarose is a polysaccharide which is extracted from red algae (gelidium).
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Gel electrophoresis is used to separate DNA fragments. Smaller the fragments, further it will move.
Option 1)
The larger the fragment size, the farther it moves
This answer is incorrect
Option 2)
The smaller the fragment size, the farther it moves
This answer is correct
Option 3)
Positively charged fragments move to farther end
This answer is incorrect
Option 4)
Negatively charged fragments do not move
This answer is incorrect