What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

  • Option 1)

    The larger the fragment size, the farther it moves

  • Option 2)

    The smaller the fragment size, the farther it moves

  • Option 3)

    Positively charged fragments move to farther end

  • Option 4)

    Negatively charged fragments do not move

 

Answers (2)

As learnt in

Gel Electrophoresis -

This technique is used to separate DNA fragments on the basis of charge and size. Agarose is a polysaccharide which is extracted from red algae (gelidium).

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 Gel electrophoresis is used to separate DNA fragments. Smaller the fragments, further it will move.


Option 1)

The larger the fragment size, the farther it moves

This answer is incorrect

Option 2)

The smaller the fragment size, the farther it moves

This answer is correct

Option 3)

Positively charged fragments move to farther end

This answer is incorrect

Option 4)

Negatively charged fragments do not move

This answer is incorrect

N neha

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