Researchers have found a novel restriction endonuclease known as "AluI." They isolated it from a bacterial species and discovered that its restriction action required ATP, Mg2+, and S-adenosyl methionine. EcoIV is classified as a restriction endonuclease.
Restriction Endonucleases of Type I
Restriction Endonucleases of Type II
Restriction Endonucleases of Type III
AluI is not related to any of the known restriction endonucleases.