An analysis of chromosomal DNA using the southern hybridization technique does not use:

  • Option 1)

    Electrophoresis

  • Option 2)

    Blotting

  • Option 3)

    Autoradiography

  • Option 4)

    PCR

 

Answers (1)

As learnt in

Southern Blotting -

This technique is developed by Edward Southern, in which DNA fragments from agarose gel are transfered to nylon membrane and then hybridization with a radio labeled probe.

- wherein

This technique is used for DNA only.

 

 Southern blotting technique doesnt't use PCR method


Option 1)

Electrophoresis

This is incorrect option

Option 2)

Blotting

This is incorrect option

Option 3)

Autoradiography

This is incorrect option

Option 4)

PCR

This is correct option

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