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Somaclones are obtained by (1) Plant breeding (2) Irradiation (3) Genetic Engineering (4) Tissue culture
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Somaclones is the term given by Larkin and Scowcroft (1981) for plant variants obtained from tissue cultures of somatic tissues. Somaclonal variation leads to the creation of additional genetic variability and is beneficial in genetic engineering for selecting and crossing plants for the creation of improved plants with desired traits. So, the correct answer is 'Tissue culture'.