Somatic hybridisation is accomplished by
Grafting between two varieties
Fusion of two protoplast of different varieties of plants
Chromosome doubling in androgenic culture
Recombinant DNA technology
As we learnt ,
Methods of breeding for disease resistance crops -
Somaclonal Variation
- wherein
Disease resistant soma clonal variants can be obtained in the following two ways, firstly , plants regenerated from cultured cells or their progeny are subjected to disease test and resistant plants are isolated. Secondly, cultured cells are selected for resistance to the toxin or culture filterate produced by the pathogen and plants are regenerated from the selected cell. In most cases, these plants are also resistant to the disease in question. Cell selection strategy is most likely to be successful in cases where the toxin is involved in disease development.