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Statement 1:For cloning, restriction enzymes with sticky ends are used to facilitate the insertion of DNA fragments from various sources into plasmids.

Statement 2: 

For cloning, restriction enzymes with sticky ends are used to easily identify plasmids with antibiotic tolerance.

 

Option: 1

Both statements are correct


Option: 2

Both statements are incorrect


Option: 3

Statement 1 is correct

 


Option: 4

Statement 2 is correct


Answers (1)

best_answer

A DNA ligase is used to combine two DNA segments into one in the process of cloning, which is the main application of sticky ends. By establishing hydrogen interactions between the bases of the other strand's complementary strand, sticky ends help DNA ligase ligate the DNA. The sticky edges are therefore preferable to the blunt ends.

Option  3  is the correct answer. 

Posted by

Ajit Kumar Dubey

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