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The band can only be seen under UV light, after staining with the_______.

Option: 1

Ethidium bromide


Option: 2

Mercury


Option: 3

 EDTA

 


Option: 4

Both B and C


Answers (1)

To identify fragments, the gel is stained with the intercalating dye ethidium bromide, followed by visualization under UV light. Ethidium bromide stains DNA in a concentration-dependent way, so the more DNA in a band on the gel, the more intensely it stains.

After staining the DNA with ethidium bromide, it is exposed to UV radiation. DNA is then visible as orange stripes.

Option 1 is the correct answer. 

Posted by

Sumit Saini

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