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The different steps of recombinant DNA technology are given below randomly.
(i) Isolation of DNA fragments or genes to be cloned
(ii) Introduction of recombinant DNA into a suitable cell (usually E.coli) called host (transformation)
(iii) Multiplication/expression of the introduced gene in the host
(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment
(v) Insertion of the isolated gene in a suitable plasmid vector
Which of the following represents the correct sequence of steps?
(i), (iii), (ii), (iv), (v)
(iii), (ii), (i), (v), (iv)
(i), (v), (ii), (iv), (iii)
(v), (i), (iii), (iv), (ii)
Answers (1)
As we have learnt,
Recombinant DNA technology -
It is a technology that allow the production of antigenic polypeptides of pathogen in bacteria or yeast
- wherein
Vaccines produced using this approach allow large scale production and hence greater availability for immunisation, e.g., hepatitis B vaccine produced from yeast.
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