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The figure below shows three steps (A,B,C) of polymerase chain reaction (PCR) select the options giving correct identification together with what it represents?

Options:

Option: 1

A - Denaturation at a temperature of about 50oC


Option: 2

C- Extension in the presence of heat stable DNA polymerase


Option: 3

A - Annealing with two sets of primers


Option: 4

B - Denaturation at a temperature of about 98oC separating the two DNA strands.


Answers (1)

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Template DNA, Primer sets, Taq polymerase, and nucleotides make up the majority of Polymerase Chain Reaction components. The cofactors required by the enzyme are also combined with the components in a tube, and the mixture is heated and cooled again to create DNA.

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Ritika Harsh

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