The figure below shows three steps (A,B,C) of polymerase chain reaction (PCR) select the options giving correct identification together with what it represents?
Options:
A - Denaturation at a temperature of about 50oC
C- Extension in the presence of heat stable DNA polymerase
A - Annealing with two sets of primers
B - Denaturation at a temperature of about 98oC separating the two DNA strands.
Template DNA, Primer sets, Taq polymerase, and nucleotides make up the majority of Polymerase Chain Reaction components. The cofactors required by the enzyme are also combined with the components in a tube, and the mixture is heated and cooled again to create DNA.