Use your analytical skills to identify the fragment that may be recognized as a cutting site by a specific restriction enzyme. Analyze the patterns and choose the option that represents the potential cutting site, revealing the secrets of DNA and the interaction of restriction enzymes
5’-GGCC-3’
3’-CCGG-5’
5’-AGTC-3’
3’-TCAG-5’
5’-AAGG-3’
3’-TTCC-5’
5’-ACCA-3’
3’-TGGT-5’
Restriction enzymes often recognize and cut DNA at specific palindromic sequences. These sequences read the same on both strands when read in opposite directions. In this case, the sequence 5’-GGCC-3’ and its complementary sequence 3’-CCGG-5’ form a palindromic sequence. This suggests that it can be recognized as a cutting site by a specific restriction enzyme.
Therefore, the fragment represented by option 1, 5’-GGCC-3’ and 3’-CCGG-5’, may be recognized as a cutting site by a specific restriction enzyme.Hence, the correct answer is option 1.