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What are the various colony phenotypes observed in terms of -galactosidase activity, AmpR gene integrity, and colony color in the recombinant pUC8? Also, how do they correlate with the presence or absence of -galactosidase activity?
Option: 1
The blue colonies exhibit an intact Ampicillin resistance gene.
Option: 2
The blue colonies are disrupted Ampicillin resistance gene.
Option: 3
The white colonies have a functional Ampicillin resistance gene.
Option: 4
The white colonies are identified where the Ampicillin resistance gene is inactivated.
Answers (1)
- As observed in the recombinant pUC8 system, white colonies exhibit a particular phenotype where the colonies which are white in color have an intact Ampicillin resistance gene.
- The absence of blue color in these white colonies indicates the absence of
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