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Which of the following enzymes is responsible for removing RNA primers and filling in the gaps with DNA nucleotides during DNA replication?

Option: 1

DNA polymerase I

Option: 2

DNA polymerase III

Option: 3

 DNA helicase

Option: 4

DNA ligase

Answers (1)


DNA polymerase III is responsible for synthesizing new DNA strands during DNA replication. It has a 5' to 3' exonuclease activity that allows it to remove RNA primers and fill in the gaps with DNA nucleotides. DNA polymerase I is also involved in this process but primarily in removing the RNA primers. DNA helicase is responsible for unwinding the double-stranded DNA, and DNA ligase is responsible for joining the Okazaki fragments on the lagging strand.

Option 2 is the correct answer. 

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