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Which of the following is a technique used to analyze gene expression?
Polymerase chain reaction (PCR)
Southern blotting
Northern blotting
Western blotting
Answers (1)
Northern blotting is a technique used to analyze gene expression by detecting the presence and quantity of specific RNA molecules in a sample. This is accomplished by separating RNA molecules by size using gel electrophoresis and then transferring the RNA to a membrane for detection using a labeled probe. PCR, Southern blotting, and Western blotting are techniques used for DNA analysis and protein detection, respectively.. Hence option 3 is the correct answer.
Option(1) Northern blotting is a technique used to analyze gene expression by detecting the presence and quantity of specific RNA molecules in a sample. Hence option 1 is incorrect.
Option(2) Northern blotting is a technique used to analyze gene expression by detecting the presence and quantity of specific RNA molecules in a sample. Hence option 2 is incorrect.
Option(4) Northern blotting is a technique used to analyze gene expression by detecting the presence and quantity of specific RNA molecules in a sample. Hence option 4 is incorrect.
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