Which of the following is not a component of the prokaryotic DNA replication machinery?
DNA polymerase III
RNA polymerase
Helicase
Topoisomerase
RNA polymerase is responsible for transcribing RNA from DNA, not replicating DNA. Prokaryotic DNA replication machinery includes several key components that work together to ensure accurate and efficient DNA replication. These components include DNA polymerase III, helicase, topoisomerase, primase, and DNA ligase. RNA polymerase, on the other hand, is responsible for transcription, which is the process of making RNA from a DNA template. Therefore, option B) RNA polymerase is not a component of the prokaryotic DNA replication machinery. Hence option 2 is the correct answer.