Which of the following is not a method of tissue culture?
Callus culture
Somatic embryogenesis
Meristem culture
Root culture
Explanation of the correct option
Root culture is not a method of tissue culture. The other options listed in the question, callus culture, somatic embryogenesis, and meristem culture, are all methods of tissue culture. Callus culture involves the growth of undifferentiated cells, somatic embryogenesis is the formation of embryos from somatic cells, and meristem culture involves the growth of the plant's apical meristem in a nutrient-rich medium. Hence option 4 is the correct answer.
Explanation of the incorrect options
Option 1 is incorrect because Callus culture is a method of tissue culture in which an undifferentiated mass of cells is grown from an explant (a piece of plant tissue). The cells in the callus can be induced to differentiate into specific cell types or can be used for genetic transformation.
Option 2 is incorrect because Somatic embryogenesis is a method of tissue culture in which embryos are formed from somatic cells (cells other than the reproductive cells). This technique is used to produce clones of plants or to induce the formation of embryos for use in genetic engineering.
Option 3 is incorrect because Meristem culture is a method of tissue culture in which the apical meristem, a region of actively dividing cells at the tip of a shoot or root, is removed from the plant and grown in a nutrient-rich medium. This technique is used for the production of virus-free plants or for the propagation of plant species that cannot be grown by other means.