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Which of the following statements is incorrect about the characteristics of a cloning vector?
 

 

 

 

 

Option: 1

Multiple restriction sites in a single vector are preferred because the vector becomes susceptible to degradation by a single restriction enzyme.


Option: 2

The vector should have an Origin of Replication (ori) so that it can replicate autonomously within the host organism.


Option: 3

The vector should be smaller in size 

 


Option: 4

The vector should have an Origin of Replication (ori) so that it can replicate autonomously within the host organism.


Answers (1)

best_answer

It is not preferred to have many restriction sites on a single vector since this makes the plasmid/vector susceptible to degradation by different restriction enzymes.

 An ideal vector typically has the following characteristics:

1) For the vector to be able to replicate itself autonomously inside the host organism, the Origin of Replication (ori) must be present.

2) The existence of several restriction sites for DNA insertion.

3) The recombinant organism should be screened using a selectable marker.

4) To be easily included in the host machinery, the vector should be reduced in size.

Option a is the correct answer. 

Posted by

Anam Khan

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