Q&A - Ask Doubts and Get Answers

Sort by :
Clear All
Q
Medical
212 Views   |  

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

  • Option 1)

    Polymerase chain reaction

  • Option 2)

    Zinc finger analysis

  • Option 3)

    Restriction enzymes

  • Option 4)

    DNA-DNA hybridization

 
As we discussed Steps of DNA fingerprinting - (a) Extraction of DNA from the sample cells. (b) Amplification of DNA by PCR (c) Restriction digestion of PCR Products (d) Separation on Agarose gel electrophoresis (e) Southern blotting (f) Hybridization using labelled VNTR probe (g) Detection by autoradiography - wherein This technique is widely used in forensic science for criminal cases    In...
Medical
91 Views   |  

Which of the following is not a feature of the plasmids?

  • Option 1)

    Independent replication

  • Option 2)

    Circular structure

  • Option 3)

    Transferable

  • Option 4)

    Single-stranded

 
  Plasmids - A plasmid is a small, circular double standard DNA molecules; has power of self replication. They usually exist in bacteria and some lower eukaryotes. They have antibiotic resistance gene. - wherein Plasmids provide genetic advantages like antibiotic resistance.    Plasmids are double stranded circular structure. Option 1) Independent replication Incorrect Option 2) Circular...
Medical
663 Views   |  

The two polypeptides of human insulin are linked together by:

  • Option 1)

    Hydrogen bonds

  • Option 2)

    Phosphodiester bond

  • Option 3)

    Covalent bond

  • Option 4)

    Disulphide bridges

 

phosphodiester bond

 

Medical
102 Views   |  

The taq polymerase enzyme is obtained from:

  • Option 1)

    Thermus aquaticus

  • Option 2)

    Thiobacillus ferroxidans

  • Option 3)

    Bacillus subtilis

  • Option 4)

    Pseudomonas putida

 
As we discussed in Taq polymerase - Are DNA polymerase, which was isolated from Thermophilous aquaticus. They are themostable DNA polymerase. Pfu->another themostable polymerase isolated from pyrococeus furiosus - wherein They can synthesize DNA at high temp () without degradation    Tag polymerase is obtained from Thermophilous aquaticus. Option 1) Thermus aquaticus Correct Option...
Medical
334 Views   |  

Which part of the tobacco plant is infected by Meloidogyne incognita?

  • Option 1)

    Flower

  • Option 2)

    Leaf

  • Option 3)

    Stem

  • Option 4)

    Root

 
As learnt in Antisense RNA - It is a single stranded RNA that is complementary to a protein coding messenger RNA (mRNA) that hybridize with it and blocks protein translation. - wherein This method is also known as Gene knock down. RNA I (Interference RNA) is also used for same purpose    Roots of the tobacco plants are infected. RNA I (Interference RNA) method is used to develop resistance...
Medical
476 Views   |  

Which of the following is a restriction endonuclease?

  • Option 1)

    Hind II

  • Option 2)

    Protease

  • Option 3)

    DNase I

  • Option 4)

    RNase

 
As learnt in Restriction Endonuclease - These enzymes cleave DNA only within or near the specific base sequence. These sequences are called as recognition sites. They are of three types RE- I, RE-II and RE-III. - wherein 1st Restriction enzyme (Hind II) was used in RDT.     Hind II are restriction endonuclease because they cleave DNA at specific sequence within DNA strand. Option 1) Hind...
Exams
Articles
Questions