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  • What are the conditions necessary for fixation of atmospheric nitrogen by Rhizobium. What is their role in N_2 -fixation?

8. What are the conditions necessary for fixation of atmospheric nitrogen by Rhizobium? What is their role in N_{2}-fixation \; ?

Answers (1)

The conditions necessary for fixation of atmospheric nitrogen by Rhizobium and their roles in nitrogen fixation are as follows: 

1. Reducing environment- A reducing environment is necessary for the action of enzyme nitrogenase which is sensitive to free oxygen.

2. Presence of nitrogenase enzyme- a Mo-Fe protein — that helps in the conversion of atmospheric free nitrogen into ammonia.

3. Presence of leghaemoglobin- The root nodules containing Rhizobium contains leg-haemoglobin, which protects nitrogenase from oxygen.

4. Source of energy as ATP- Nitrogen fixation is energy requiring process thus ATP is required.

5. Source of reducing power NAD(P), H2 or FMNH2- 

6. Ferredoxin as the electron donor- Ferredoxin makes nitrogen reactive. 

7. Magnesium ions as cofactors- Magnesium act as a cofactor and helps in the activity of nitrogenase. 

 

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Sonika

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