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Get Answers to all your Questions
Study the figure of vector pBR322 given below.
Identify A, B and C and explain their roles in cloning a vector.
Answers (1)
pBR322 is one of the first-ever plasmid, which was used for cloning. It has genes that codes for resistance to ampicillin and tetracycline.
Site A: amp
Role: This site codes for ampicillin resistance.
Site B: ori
Role: It is the site where DNA replication begins which enables the plasmid to reproduce itself to survive within the cell.
Site C: rop Role:
rop stands for “regulator of origin of plasmid” this site is involved in the regulation of copy of a number of pBR322.
Site D: tet Role: This site code for tetracycline resistance.
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