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  • Help me answer: In genetic engineering, the antibiotics are used:

In genetic engineering, the antibiotics are used:

  • Option 1)

    to select healthy vectors

  • Option 2)

    as sequences from where replication starts

  • Option 3)

    to keep the cultures free of infection

  • Option 4)

    as selectable markers

 

Answers (1)

best_answer

As learnt in

Selection of vector -

Based on:

  • size of the gene of interest
  • presence of ori size 
  • copy number of plasmid
  • restriction enzyme sites
  • antibiotic resistance genes as a selectable markers  

- wherein

The copy number of plasmid decides the power of replication inside the host. Usually, high copy number (>100) is used to express the protein. 

 

 Antibiotics are used as selectable markers


Option 1)

to select healthy vectors

This is incorrect answer

Option 2)

as sequences from where replication starts

This is incorrect option

Option 3)

to keep the cultures free of infection

This is incorrect option

Option 4)

as selectable markers

This is correct option

Posted by

divya.saini

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