During glycolysis, which enzyme converts glucose-6-phosphate into fructose-6-phosphate?
Hexokinase
Isomerase
Phosphofructokinase
Aldolase
During the second step of glycolysis, an isomerase enzyme converts Glucose-6-phosphate into Fructose-6-phosphate. Isomerases are enzymes that catalyze the conversion of a molecule into its isomer, which has the same chemical formula but a different structural arrangement.
Hexokinase (option 1) is incorrect because it catalyzes the phosphorylation of glucose, not the conversion of glucose-6-phosphate into fructose-6-phosphate.
Phosphofructokinase (option 3) is incorrect because it is responsible for the phosphorylation of fructose-6-phosphate into fructose-1,6-bisphosphate.
Aldolase (option 4) is incorrect because it leaves fructose-1,6-bisphosphate in two three-carbon isomers.
Therefore, the correct answer is option 2 - Isomerase.