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  • During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates outOption: 1 RNA<div class='qn

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

Option: 1

RNA


Option: 2

DNA


Option: 3

Histones


Option: 4

Polysaccharides


Answers (1)

best_answer

The correct option is Option 2) DNA.

During the purification process in recombinant DNA technology, chilled ethanol is often used to precipitate out DNA. This technique is based on the principle that DNA is less soluble in cold ethanol than in aqueous solutions.

When chilled ethanol is added to the DNA solution, it causes the DNA molecules to come out of solution and form a visible precipitate. This is because the ethanol disrupts the hydrogen bonding and hydrophobic interactions that hold the DNA molecules together, leading to their aggregation and precipitation.

By centrifuging the mixture, the precipitated DNA can be collected as a pellet, while other contaminants remain in the supernatant. The DNA pellet can then be further purified and processed for various applications in recombinant DNA technology.

Therefore, Option 2) DNA is the correct answer for the component that precipitates out when chilled ethanol is added during the purification process for recombinant DNA technology.

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Sayak

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