S - phase (DNA synthesis phase):
- Following G1, the cell enters the S stage, when DNA synthesis or replication occurs.
- At the beginning of the S stage, each chromosome is composed of one DNA double helix.
- Following DNA replication, each chromosome is composed of two identical DNA double helix molecules.
- Each double helix is called a chromatid.
- Another way of expressing these events is to say that DNA replication has resulted in duplicated chromosomes, and the two chromatids will remain attached until they are separated during mitosis.
- If the initial amount of DNA is denoted as 2C then it increases to 4C.
- However, there is no increase in the chromosome number; if the cell had diploid or 2n number of chromosomes at G1, even after S phase the number of chromosomes remains the same, i.e., 2n.

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The amount of DNA in a cell remain at 4C level if the initial amount is denoted as 2C during G2 only because during M phase, the DNA would be equally divided between the daughter cells, restoring the 2C level.
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