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Q.95) Given below are two statements :

Statement I : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below :

A)  Statement I is incorrect but statement II is correct

B)  Both statement I and statement II are correct

C) Both statement I and statement II are incorrect

D)  Statement I is correct but statement II is incorrect<

 

Answers (1)

best_answer

Answer: Correct answer is option(4), Statement I is correct but statement II is incorrect

Explanation: 

  • Statement I is correct: The DNA fragments separated by gel electrophoresis can indeed be used in the construction of recombinant DNA. Once the fragments are separated, they can be isolated, purified, and inserted into vectors to create recombinant DNA molecules.
     
  • Statement II is incorrect: In gel electrophoresis, smaller DNA fragments move faster and thus migrate further towards the anode (positive side). Conversely, larger DNA fragments move more slowly and are found closer to the wells (negative side) because they face more resistance while migrating through the gel.
    Hence, the correct answer is option (4): Statement I is correct but statement II is incorrect.

 

Posted by

Parbin Huda Baruah

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