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I need help with An analysisof chromosomal DNA using Southern hybridization technique does not use:

An analysis of chromosomal DNA using Southern hybridization technique does not use:

  • Option 1)

    Electrophoresis

  • Option 2)

    Blotting

  • Option 3)

    Autoradiography

  • Option 4)

    PCR

 
Answers (1)
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As we learnt in

Southern Blotting -

This technique is developed by Edward Southern, in which DNA fragments from agarose gel are transfered to nylon membrane and then hybridization with a radio labeled probe.

- wherein

This technique is used for DNA only.

 

 and

Gel Electrophoresis -

This technique is used to separate DNA fragments on the basis of charge and size. Agarose is a polysaccharide which is extracted from red algae (gelidium).

-

 

 


Option 1)

Electrophoresis

Correct

Option 2)

Blotting

Incorrect

Option 3)

Autoradiography

Incorrect

Option 4)

PCR

Incorrect

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