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Proton gradient across the thylakoid membrane is created due to

Option: 1

Decrease in the number of protons in stroma and increase in that in lumen


Option: 2

Increase in the number of protons in stroma and decrease in that in lumen


Option: 3

Increase in the number of protons in stroma and lumen both


Option: 4

Decrease in the number of protons in both stroma and lumen


Answers (1)

best_answer
 

What causes proton gradient across the membrane?

  • Since the splitting of the water molecule takes place on the inner side of the membrane, the protons or hydrogen ions that are produced by the splitting of water accumulate within the lumen of the thylakoids.
  • As electrons move through the photosystems, protons are transported across the membrane. 
  • This happens because the primary acceptor of the electron which is located towards the outer side of the membrane transfers its electron not to an electron carrier but to an H carrier. 
  • Hence, this molecule removes a proton from the stroma while transporting an electron.
  • When this molecule passes on its electrons to the electron carrier on the inner side of the membrane, the proton is released into the inner side or the lumen side of the membrane.
  • The NADP reductase enzyme is located on the stroma side of the membrane. Along with electrons that come from the acceptor of electrons of PS I, protons are necessary for the reduction of NADP+ to NADPH.H+. These protons are also removed from the stroma.
  • Hence, within the chloroplast, protons in the stroma decrease in number, while in the lumen, there is an accumulation of protons. This creates a proton gradient across the thylakoid membrane as well as a measurable decrease in pH in the lumen.

A proton gradient across the thylakoid membrane is created when the number of protons decrease in stroma and increases in lumen. Hence, the correct answer is option a.

 

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Gaurav

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