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  • Restriction endonuclease cleaves DNA molecules at specific ‘recognition sites': One such enzyme has four recognition sites on a circular DNA molecule. After complete digestion how many fr

Restriction endonuclease cleaves DNA molecules at specific ‘recognition sites': One such enzyme has four recognition sites on a circular DNA molecule. After complete digestion how many fragments would be produced upon reaction with the enzyme?

Option: 1

4


Option: 2

5


Option: 3

3


Option: 4

6


Answers (1)

best_answer

 If a restriction endonuclease cleaves a circular DNA molecule at four specific recognition sites, it will produce a total of four fragments upon complete digestion.

Each recognition site will be cleaved by the restriction enzyme, resulting in two ends. With four recognition sites, there are a total of four cleavage events, and each cleavage produces two ends. Therefore, the total number of fragments produced is equal to the number of cleavage events, which is 4.

Since the DNA molecule is circular, there is no separate circular fragment generated upon digestion. All four fragments produced would be linear fragments.

Hence, the correct answer is option 1. 

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Riya

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