Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

  • Option 1)

    5'..................... CGTTCG.............3'

    3'......................ATGGTA...............5'

  • Option 2)

    5'..................... GATATG.............3'

    3'......................CTACTA...............5'

  • Option 3)

    5'..................... GAATTC.............3'

    3'......................CTTAAG...............5'

  • Option 4)

    5'..................... CACGTA.............3'

    3'......................CTCAGT...............5'

 

Answers (1)

As learnt in

Palindromic sequence for ECORI -

- wherein

produce sticky ends

 

 


Option 1)

5'..................... CGTTCG.............3'

3'......................ATGGTA...............5'

This option is incorrect

Option 2)

5'..................... GATATG.............3'

3'......................CTACTA...............5'

This option is incorrect

Option 3)

5'..................... GAATTC.............3'

3'......................CTTAAG...............5'

This option is correct

Option 4)

5'..................... CACGTA.............3'

3'......................CTCAGT...............5'

This option is incorrect

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