What is the main technique used in ICSI?
Intrauterine insemination
In vitro fertilization
Direct injection of sperm into the egg/ovum
Gamete intrafallopian transfer
The main technique used in Intracytoplasmic Sperm Injection (ICSI) is direct injection of sperm into the egg/ovum. This involves using a micro-needle to inject a single sperm cell directly into the cytoplasm of the egg/ovum to achieve fertilization. The technique is used to treat male factor infertility, where the male partner has low sperm count, poor sperm motility, or poor sperm quality. Hence option 3 is the correct answer.