Q.167) Which of the following are the posttranscriptional events in an eukaryotic cell?
A. Transport of pre-mRNA to cytoplasm prior to splicing.
B. Removal of introns and joining of exons.
C. Addition of methyl group at 5′5^{\prime}5′ end of hnRNA.
D. Addition of adenine residues at 3' end of hnRNA.
E. Base pairing of two complementary RNAs.
A) C, D, E only
B) A, B, C only
C) B, C, D only
D) B, C, E only
Q.167) In eukaryotic cells, the initial RNA transcript (hnRNA) undergoes several posttranscriptional modifications before becoming functional mRNA. These include:
Removal of introns and joining of exons (splicing) (B)
Addition of a methylated cap at the 5′5'5′ end (C)
Addition of a poly-A tail (adenine residues) at the 3′3'3′ end (D)
Statement A is incorrect because splicing occurs in the nucleus before the RNA is transported to the cytoplasm.
Statement E is unrelated to typical mRNA processing — it refers to RNA-RNA interactions like in RNA interference.
Hence, the correct answer is option 3) B, C, D only