Q.114) Which of the following statements about RuBisCO is true?
A) It catalyzes the carboxylation of RuBP.
B) It is active only in the dark.
C) It has higher affinity for oxygen than carbon dioxide.
D) It is an enzyme involved in the photolysis of water.
Q.114) Option (1) is correct:
RuBisCO (Ribulose bisphosphate carboxylase/oxygenase) is the key enzyme involved in the calvin cycle (dark reaction) of photosynthesis. It catalyzes the carboxylation of ribulose bisphosphate (RuBP), fixing carbon dioxide (CO?) to RuBP and forming two molecules of 3-phosphoglycerate (3-PGA). This is the first step in carbon fixation during photosynthesis.
Option (2) is incorrect:
RuBisCO is not only active in the dark. It is involved in the Calvin cycle, which occurs in the stroma of chloroplasts and is independent of light, though it indirectly depends on the products of light reactions (ATP and NADPH).
Option (3) is incorrect:
RuBisCO has a higher affinity for carbon dioxide (CO?) than for oxygen (O?). However, it can also bind to oxygen, leading to photorespiration, but its affinity for CO? is greater under normal conditions.
Option (4) is incorrect:
RuBisCO is not involved in the photolysis of water. The photolysis of water is carried out by another enzyme, water-splitting complex (part of Photosystem II), which helps release oxygen in the light-dependent reactions.
Hence, the correct answer is option (1): It catalyzes the carboxylation of RuBP.