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  • A transverse section of stem is stained first with safranin and then with fast green following the usual schedule of double staining for the preparation of a permanent slide. What would be the colour of the stained xylem and phloem?

A transverse section of stem is stained first with safranin and then with fast green following the usual schedule of double staining for the preparation of a permanent slide. What would be the colour of the stained xylem and phloem?

  1. Red and green
  2. Green and red
  3. Orange and yellow
  4. Purple and orange

 

Answers (1)

(a) Red and green

Explanation: The xylem is coloured red with safranin because the walls of the xylem cells contain lignin which picks up the red dye of safranin and phloem is coloured green with fast green because fast green stains the dense cytoplasm and cell walls present in the phloem.

 

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