ATP produced during glycolysis is a result of substrate-level phosphorylation. Explain.
Substrate-level phosphorylation is the stage where the ATP formation happens by the transferring of the phosphoryl (PO3) group from a phosphorylated reactive intermediate to ADP. In the process of glycolysis, ATP formation happens at two main stages which are as follows:
1) Conversion of BPGA (bisphosphoglyceric acid) into PGA (phosphoglyceric acid).
2) Conversion of PEP (phosphoenolpyruvate) into pyruvic acid.