9. Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?

Answers (1)
S Sonika

In recombinant DNA technology selection of transformed and non transformed cells can be done using reporter genes that encode for reporter enzymes. During the RDT experiment, the foreign gene is joined with a reporter gene. The reporter gene should be such that it produces a visible expression. For example, Lac Z gene which codes for enzyme beta-galactosidase is used as a reporter gene. The activity of this gene is not found in transformed cells as the product formed by its catalysation is not formed in transformed cells and bacterial colonies appear white. In non-transformed cells, this gene shows its activity and the catalysed product is formed, as a result of this, bacterial colonies appear blue. Thus, reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker.

Preparation Products

JEE Main Rank Booster 2021

This course will help student to be better prepared and study in the right direction for JEE Main..

₹ 13999/- ₹ 9999/-
Buy Now
Rank Booster NEET 2021

This course will help student to be better prepared and study in the right direction for NEET..

₹ 13999/- ₹ 9999/-
Buy Now
Knockout JEE Main April 2021 (Easy Installments)

An exhaustive E-learning program for the complete preparation of JEE Main..

₹ 4999/-
Buy Now
Knockout NEET May 2021

An exhaustive E-learning program for the complete preparation of NEET..

₹ 22999/- ₹ 14999/-
Buy Now
Knockout NEET May 2022

An exhaustive E-learning program for the complete preparation of NEET..

₹ 34999/- ₹ 24999/-
Buy Now
Exams
Articles
Questions