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(a) Explain the different steps carried out in Polymerase Chain Reaction, and the specific roles of the enzymes used.

(b) Mention application of PCR in the field of

(i) Biotechnology
(ii) Diagnostics

 

 

 

 
 
 
 
 

Answers (1)

a) Different steps carried out in Polymerase Chain Reaction, and the specific roles of the enzymes are shown in the given flow chart diagram:

b) Application of PCR in the field of:

(i) Biotechnology

  • Multiple copies of the gene of interest can be made through In vitro process
  • gene amplification

(ii) Diagnostics

  • Early detection of disease at a time when the symptoms are not yet visible
  • can detect mutations in genes in suspected cancer patients
  • a powerful technique to identify many other genetic disorders
Posted by

Safeer PP

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