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  • (a) Mention the importance of gel-electrophoresis in biotechnology. (b) Explain the process of this technique.      

(a) Mention the importance of gel-electrophoresis in biotechnology.

(b) Explain the process of this technique.

 

 

 

 
 
 
 
 

Answers (1)

a) The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. And we can extract our desired fragment based on its molecular size.

b) 1. DNA fragments are negatively-charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. (agarose).

2. The DNA fragments separate (resolve) according to their size through the sieving effect provided by the agarose gel. Hence, the smaller the fragment size, the farther it moves.

3. The separated DNA fragments can be visualized only after staining the     DNA with a compound known as ethidium bromide followed by exposure     to UV radiation

4. Bright orange coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light are seen.

5. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.

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Priyanka Kumari

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