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(a) Name the selectable markers in the cloning vector pBR322? Mention the role they play.
(b) Why is the coding sequence of an enzyme a preferred selectable marker in comparison to the ones named above?
Answers (1)
(a) ampR/ ampicillin resistance genes, tetR/ tetracycline resistance gene.
They help in identifying and eliminating non - transformants / non - recombinants and selectively permitting the growth of the transformants/recombinants.
(b) Simpler process, less cumbersome, in the presence of chromogenic substrate recombinants are colourless and non - recombinants are blue in colour.
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