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  • Explain the mechanism of DNA replication with the help of a replication fork. What role does the enzymes DNA-ligase play in a DNA replication fork?      

Explain the mechanism of DNA replication with the help of a replication fork. What role does the enzymes DNA-ligase play in a DNA replication fork?

 

 

 

 
 
 
 
 

Answers (1)

The process of replication requires a set of enzymes. The main enzyme is DNA - dependent DNA polymerase, since, it uses DNA template to catalyse the polymerisation of deoxynucleotides these polymerase are highly efficient, fast and also catalyse the reaction with high degree of accuracy. Any mistake during replication would result into mutations. Deoxyribonucleotides serve dual purpose. In addition to acting as substrates, they provide energy for polymeisation of reaction.

For long DNA molecules, since the two strands of DNA cannot be seprated in its entire length (due to high energy requirement the replication occur within a small opening of the DNA helix, referred to as replication fork.

The DNA dependent DNA polymerase catalyse polymeisation only in one direction 5'→3'. This creates some additional complications at the replicating fork. Consequently on one strand ,( template with poplarity 3'→5'), replication does not initate randomly at any one place, it starts at the origin of replication.

DNA liagase joins the discontinously synthesised fragments by catalysing the formation of phosphodiester bond.

 

Posted by

Priyanka Kumari

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