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Write the role of the following sites in pBR322 cloning vector :
(a) rop
(b) ori
(c) selectable marker
Answers (1)
a) rop- rop stands for “regulator of origin of plasmid”. This site is involved in the regulation of copy of the number of pBR322.
b) ori- ori stands for origin of replication. It is the site where DNA replication begins which enables the plasmid to reproduce itself to survive within the cell.
c) selectable marker- In addition to ‘ori’, the vector requires a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
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