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- Which of the following statements is true regarding the Biuret test?Option: 1 The intensity of the color change is proportional to the concentration
Which of the following statements is true regarding the Biuret test?
The intensity of the color change is proportional to the concentration of proteins in the sample.
The Biuret reagent reacts specifically with the amino acid lysine.
The Biuret test is used to detect the presence of reducing sugars in a sample.
The Biuret test is only applicable to proteins with a specific sequence of amino acids.
Answers (1)
The Biuret test is a common assay used to detect the presence of proteins in a sample. When the Biuret reagent is added to a protein-containing solution, it reacts with the peptide bonds in the protein, forming a complex that results in a color change from blue to violet. The intensity of the color change is directly proportional to the concentration of proteins in the sample, thus making it a useful tool for protein quantification. Option 1) correctly identifies this property of the Biuret test.
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