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Assertion: Eli Lily utilized two DNA sequences corresponding to the A and B chains of human insulin, which were introduced into an E. coli plasmid to generate polypeptide chains of insulin.
Reason: The A and B chains of insulin were synthesized individually, extracted separately, and subsequently combined through the creation of disulfide bonds to form the complete human insulin molecule.
Both the assertion and reason are true, and the reason is a correct explanation of the assertion.
Both the assertion and reason are true, but the reason is not a correct explanation of the assertion.
The assertion is true, but the reason is false.
Both the assertion and reason are false.
Answers (1)
Option 2 is the correct answer. Both the assertion and reason are true. However, the reason is not a correct explanation of the assertion. While Eli Lily did introduce DNA sequences corresponding to the A and B chains of human insulin into E. coli to produce polypeptide chains, the reason incorrectly states that the A and B chains were combined solely through the creation of disulfide bonds. In reality, the A and B chains are produced separately and combined through a process called refolding, where they undergo proper folding and disulfide bond formation to form the complete human insulin molecule.
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