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Assertion: The presence of multiple restriction sites within the vector is considered undesirable.
Reason: This leads to the generation of numerous fragments, thereby complicating the overall process.
If both Assertion & Reason are True &the Reason is a correct explanation of the Assertion.
If both Assertion & Reason are True but Reason is not a correct explanation of the Assertion.
If Assertion is True but the Reason is False.
If both Assertion & Reason are false.
Answers (1)
The presence of multiple restriction sites within a vector can actually be desirable in many cases. Multiple restriction sites provide flexibility in the cloning process, allowing for the insertion of different DNA fragments at different sites within the vector. It can simplify the process of constructing recombinant DNA molecules by providing more options for inserting desired fragments.
The reason stated, which suggests that the generation of numerous fragments complicates the overall process, is also false. The number of fragments generated during the DNA manipulation process does not inherently complicate the process. The complexity mainly arises from factors such as fragment size, proper identification and isolation of the desired fragment, and efficient ligation of fragments into the vector.
Therefore, the correct answer is option 4 - If both Assertion & Reason are false.
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