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By employing precise laboratory methodologies, a scientist embarked on the intricate task of transferring Gene X from a plasmid exclusive to bacteria to a plasmid exclusive to yeast. The initial step involved the strategic utilization of restriction enzymes to cleave, or fragment, the bacteria-specific plasmid on both sides of the target gene. Consequently, this enzymatic process generated a heterogeneous mixture consisting of two linear DNA fragments
The bacterial plasmid is shown in a diagram before and after being digested by a restriction enzyme (bp = base pairs).
The researcher divided the obtained DNA fragments by size using gel electrophoresis. Then, in order to separate the gene from the gel, the scientist removed the portion of the gel that contained Gene X.
The digested plasmid DNA is depicted in the accompanying diagram using gel electrophoresis, with distinct bands denoted by the letters A, B, and C. Which band was the scientist able to separate Gene X DNA from?
A Band
B Band
C Band
Both A and B bands
Answers (1)
In order to determine which band was removed by the scientist to isolate Gene X DNA, we need to identify the band that corresponds to a DNA fragment of approximately 1,890 base pairs (bp) in length. Comparing the digested plasmid bands with the bands of a DNA ladder, which provide known sizes, we can observe that the lower band in the sample is likely the one around 1,890 bp. Therefore, it can be concluded that the scientist cut out Band B to isolate Gene X DNA.Hence, the correct answer is option 2.
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