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By performing restriction enzyme digestions on a 4 kb DNA molecule with various enzymes, the following fragment sizes were obtained: digestion with EcoRI produced 1 kb and 3 kb fragments, digestion with HindIII produced 1.5 kb and 2.5 kb fragments, and digestion with both EcoRI and HindIII together produced 0.5 kb, 1 kb, and 2.5 kb fragments. From this information, which of the following enzyme sites is flanking the 0.5 kb fragment?
PstI-HindIII
EcoRI-PstI
EcoRI
EcoRI-HindIII
Answers (1)
Based on the information provided, the digestion of the 4 kb DNA molecule with EcoRI yields two fragments of 1 kb and 3 kb each. This indicates that there is an EcoRI recognition site within the DNA molecule. Additionally, the digestion with HindIII produces fragments of 1.5 kb and 2.5 kb. This suggests the presence of a HindIII recognition site within the DNA molecule as well.
When the DNA molecule is digested with both EcoRI and HindIII together, fragments of 0.5 kb, 1 kb, and 2.5 kb are obtained. Since the 0.5 kb fragment is produced only when both EcoRI and HindIII are used, it implies that this fragment is flanked by the recognition sites of both enzymes. Therefore, the 0.5 kb fragment is flanked by the EcoRI-HindIII enzyme sites.Hence, the correct answer is option 4.
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