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  • Choose the option that represents the minimal set of elements needed for a plasmid to function as a cloning vector.Option: 1

Choose the option that represents the minimal set of elements needed for a plasmid to function as a cloning vector.

Option: 1

Ori, multiple cloning site, promoter


Option: 2

Ori, multiple cloning site, a selectable marker, promoter


Option: 3

Ori, multiple cloning site, a selectable marker, translation start site 


Option: 4

Ori, multiple cloning site, selectable marker


Answers (1)

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For a plasmid to be a cloning vector, it requires an origin of replication (Ori) to enable autonomous replication within the host cell. The multiple cloning site (MCS) provides various unique restriction enzyme recognition sites for the insertion of foreign DNA fragments. The selectable marker allows for the identification and selection of cells that have successfully taken up the plasmid.

The presence of a promoter or translation start site is not essential for a plasmid to function as a cloning vector. These elements are relevant for gene expression but not specifically required for cloning purposes.

Therefore, the minimum essential elements for a plasmid to serve as a cloning vector are the origin of replication (Ori), multiple cloning site (MCS) and a selectable marker, as given in option 4.

Posted by

Irshad Anwar

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